Description: Mouse monoclonal antibody to Synaptophysin Immunogen: Purified recombinant fragment of SYP expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: SYP Alternative Names: Synaptophysin; Major synaptic vesicle protein p38Entrez Gene (Human): 6855Entrez Gene (Mouse) : 20977Entrez Gene (Rat) : 24804SwissProt (Human): P08247SwissProt (Mouse) : Q62277SwissProt (Rat) : P07825Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates.
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  Immunohistochemical analysis of Synaptophysin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.