Description: Mouse monoclonal antibody to Metadherin Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/50 - 1/100) Gene Symbol: MTDH Alternative Names: AEG1; LYRIC; Protein LYRIC; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion proteinEntrez Gene (Human): 92140SwissProt (Human): Q86UE4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Metadherin expression in Jurkat (A), A549 (B), MCF7 (C), Hela (D) whole cell lysates.
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  Immunohistochemical analysis of Metadherin staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Flow cytometric analysis of Metadherin in Hela cells (green) and negative control (purple).