Description: Mouse monoclonal antibody to LIN28A Immunogen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: LIN28A Alternative Names: CSDD1; LIN28; ZCCHC1; Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1Entrez Gene (Human): 79727SwissProt (Human): Q9H9Z2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of LIN28A expression in NTERA2 (A) whole cell lysates.
-
Immunofluorescent analysis of LIN28A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.