Description: Mouse monoclonal antibody to EPHB1 Immunogen: Purified recombinant fragment of EphB1 (aa19-133) expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EPHB1 Alternative Names: ELK; EPHT2; HEK6; NET; Ephrin type-B receptor 1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; Tyrosine-protein kinase receptor EPH-2Entrez Gene (Human): 2047SwissProt (Human): P54762Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EPHB1 expression in MDAMB468 (A), MDAMB453 (B), MCF7 (C), T47D (D), SKBR3 (E) whole cell lysates.
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  Immunohistochemical analysis of EPHB1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.