Description: Mouse monoclonal antibody to BID Immunogen: Purified recombinant fragment of human BID expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), FC (1/50 - 1/100) Gene Symbol: BID Alternative Names: BH3-interacting domain death agonist; p22 BID; BIDEntrez Gene (Human): 637SwissProt (Human): P55957Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BID expression in 293T (A), Jurkat (B), A431 (C) whole cell lysates.
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  Immunofluorescent analysis of BID staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.