Description: Mouse monoclonal antibody to EIF2S1 Immunogen: Purified recombinant human eIF2α protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: EIF2S1 Alternative Names: EIF2A; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alphaEntrez Gene (Human): 1965Entrez Gene (Mouse) : 13665Entrez Gene (Rat) : 54318SwissProt (Human): P05198SwissProt (Mouse) : Q6ZWX6SwissProt (Rat) : P68101Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EIF2S1 expression in K562 (A), COS7 (B), NIH3T3 (C), C2C12 (D), MCF7 (E), C6 (F), Hela (G) whole cell lysates.
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Immunofluorescent analysis of EIF2S1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.