Description: Mouse monoclonal antibody to E2F1 Immunogen: Purified recombinant human E2F-1 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: E2F1 Alternative Names: RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1Entrez Gene (Human): 1869Entrez Gene (Mouse) : 13555SwissProt (Human): Q01094SwissProt (Mouse) : Q61501SwissProt (Rat) : O09139Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of E2F1 expression in C6 (A), Raw264.7 (B), Hela (C) whole cell lysates.
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Immunofluorescent analysis of E2F1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.