Description: Mouse monoclonal antibody to DDX3X Immunogen: Purified recombinant human DDX3 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50) Gene Symbol: DDX3X Alternative Names: DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3 X-chromosomal; DEAD box X isoform; Helicase-like protein 2; HLP2Entrez Gene (Human): 1654Entrez Gene (Mouse) : 13205SwissProt (Human): O00571SwissProt (Mouse) : Q62167Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DDX3X expression in Hela (A), NIH3T3 (B), C6 (C), COS7 (D), K562 (E), Jurkat (F) whole cell lysates.
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Immunofluorescent analysis of DDX3X staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.