Description: Mouse monoclonal antibody to HSP90 beta Immunogen: Purified recombinant human Hsp90 beta protein fragments expressed in E.coli Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: HSP90AB1 Alternative Names: HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84Entrez Gene (Human): 3326Entrez Gene (Mouse) : 15516Entrez Gene (Rat) : 301252SwissProt (Human): P08238SwissProt (Mouse) : P11499SwissProt (Rat) : P34058Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of HSP90 beta expression in Hela (A), NIH3T3 (B), C6 (C), CHOK1 (D), COS7 (E) whole cell lysates.
-
Immunofluorescent analysis of HSP90 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.