Description: Mouse monoclonal antibody to WDR77 Immunogen: Purified recombinant human WDR77 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: WDR77 Alternative Names: MEP50; WD45; Methylosome protein 50; MEP-50; Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50Entrez Gene (Human): 79084Entrez Gene (Mouse) : 70465Entrez Gene (Rat) : 310769SwissProt (Human): Q9BQA1SwissProt (Mouse) : Q99J09SwissProt (Rat) : Q4QR85Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of WDR77 expression in C6 (A), NIH3T3 (B), K562 (C) whole cell lysates.
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Immunofluorescent analysis of WDR77 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.