Description: Mouse monoclonal antibody to RPA1 Immunogen: Purified recombinant human RPA70 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: RPA1 Alternative Names: REPA1; RPA70; Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding proteinEntrez Gene (Human): 6117Entrez Gene (Mouse) : 68275SwissProt (Human): P27694SwissProt (Mouse) : Q8VEE4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RPA1 expression in Hela (A), A431 (B), MCF7 (C), COS7 (D), HT1080 (E), K562 (F) whole cell lysates.
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Immunofluorescent analysis of RPA1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.