Description: Mouse monoclonal antibody to Focal Adhesion Kinase Immunogen: Purified recombinant human FAK protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: PTK2 Alternative Names: FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAKEntrez Gene (Human): 5747SwissProt (Human): Q05397Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Focal Adhesion Kinase expression in COS7 (A), HUVEC (B), A549 (C), Hela (D) whole cell lysates.
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Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).