Description: Mouse monoclonal antibody to CDK5 Immunogen: Purified recombinant human CDK5(N-terminus) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: CDK5 Alternative Names: CDKN5; Cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunitEntrez Gene (Human): 1020Entrez Gene (Mouse) : 12568Entrez Gene (Rat) : 140908SwissProt (Human): Q00535SwissProt (Mouse) : P49615SwissProt (Rat) : Q03114Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CDK5 expression in NIH3T3 (A), Hela (B), PC12 (C), COS7 (D), Jurkat (E), K562 (F) whole cell lysates.
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  Immunofluorescent analysis of CDK5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.