Description: Mouse monoclonal antibody to TORC1 Immunogen: Purified recombinant human MECT1 / Torc1 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100) Gene Symbol: CRTC1 Alternative Names: KIAA0616; MECT1; TORC1; WAMTP1; CREB-regulated transcription coactivator 1; Mucoepidermoid carcinoma translocated protein 1; Transducer of regulated cAMP response element-binding protein 1; TORC-1; Transducer of CREB protein 1Entrez Gene (Human): 23373SwissProt (Human): Q6UUV9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TORC1 expression in Hela (A), Jurkat (B) whole cell lysates.
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Immunofluorescent analysis of TORC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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Flow cytometric analysis of TORC1 in K562 cells (red). Blue line histogram represents the isotype control.