Description: Mouse monoclonal antibody to SMAD5 Immunogen: Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), FC (1/50 - 1/100) Gene Symbol: SMAD5 Alternative Names: MADH5; Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5Entrez Gene (Human): 4090SwissProt (Human): Q99717Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of SMAD5 expression in Hela (A), Jurkat (B), K562 (C) whole cell lysates.
-
Immunofluorescent analysis of SMAD5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
-
Flow cytometric analysis of SMAD5 in Jurkat cells (red). Black line histogram represents the isotype control.