Description: Mouse monoclonal antibody to HSP70 Immunogen: Purified recombinant human Hsp70(N-terminus) protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: HSPA1A Alternative Names: HSP72; HSPA1; HSX70; Heat shock 70 kDa protein 1A; Heat shock 70 kDa protein 1; HSP70-1; HSP70.1Entrez Gene (Human): 3303SwissProt (Human): P0DMV8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSP70 expression in Hela (A), HepG2 (B) whole cell lysates.
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Immunofluorescent analysis of HSP70 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.