Description: Mouse monoclonal antibody to KDM5C Immunogen: Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: KDM5C Alternative Names: DXS1272E; JARID1C; SMCX; XE169; Lysine-specific demethylase 5C; Histone demethylase JARID1C; Jumonji/ARID domain-containing protein 1C; Protein SmcX; Protein Xe169Entrez Gene (Human): 8242SwissProt (Human): P41229Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of KDM5C expression in Hela (A), 293T (B), T47D (C), MCF7 (D) whole cell lysates.
-
Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.