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Anti-Visfatin Antibody CQA8218
  • Western blot analysis of Visfatin expression in NIH3T3 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates.
  • Immunohistochemical analysis of Visfatin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Visfatin staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Product NameAnti-Visfatin Antibody
Cat No: CQA8218
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH, IF/IC, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit monoclonal antibody to Visfatin
Immunogen: A synthetic peptide of human Visfatin
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Monoclonal
Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
Gene Symbol: NAMPT
Alternative Names: PBEF; PBEF1; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin
Entrez Gene (Human): 10135
Entrez Gene (Mouse) : 59027
Entrez Gene (Rat) : 297508
SwissProt (Human): P43490
SwissProt (Mouse) : Q99KQ4
SwissProt (Rat) : Q80Z29
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Visfatin expression in NIH3T3 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates.
  • Immunohistochemical analysis of Visfatin staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of Visfatin staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
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