Description: Rabbit monoclonal antibody to Thrombospondin 1 Immunogen: Recombinant protein of human Thrombospondin 1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: THBS1 Alternative Names: TSP; TSP1; Thrombospondin-1Entrez Gene (Human): 7057SwissProt (Human): P07996SwissProt (Mouse) : P35441Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Thrombospondin 1 expression in MCF7 (A), 293T (B), C2C12 (C) whole cell lysates.
• 
  Immunohistochemical analysis of Thrombospondin 1 staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.