Description: Rabbit monoclonal antibody to STAT5B Immunogen: Recombinant protein of human STAT5b Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: STAT5B Alternative Names: Signal transducer and activator of transcription 5BEntrez Gene (Human): 6777Entrez Gene (Mouse) : 20851Entrez Gene (Rat) : 25126SwissProt (Human): P51692SwissProt (Mouse) : P42232SwissProt (Rat) : P52632Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of STAT5B expression in Hela (A), A549 (B), HL60 (C), Raw264.7 (D), PC12 (E) whole cell lysates.
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  Immunohistochemical analysis of STAT5B staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of STAT5B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.