Description: Rabbit monoclonal antibody to KEAP1 Immunogen: Recombinant protein of human Keap1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: KEAP1 Alternative Names: INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19Entrez Gene (Human): 9817Entrez Gene (Mouse) : 50868SwissProt (Human): Q14145SwissProt (Mouse) : Q9Z2X8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of KEAP1 expression in Raw264.7 (A), Hela (B), A549 (C), C2C12 (D) whole cell lysates.
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Immunohistochemical analysis of KEAP1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.