Description: Rabbit monoclonal antibody to HSP90 beta Immunogen: A synthetic peptide of human Hsp90 beta Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HSP90AB1 Alternative Names: HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84Entrez Gene (Human): 3326Entrez Gene (Mouse) : 15516Entrez Gene (Rat) : 301252SwissProt (Human): P08238SwissProt (Mouse) : P11499SwissProt (Rat) : P34058Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HSP90 beta expression in PC12 (A), Raw264.7 (B), Hela (C), A549 (D), HL60 (E) whole cell lysates.
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  Immunohistochemical analysis of HSP90 beta staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.