Description: Rabbit monoclonal antibody to HIF1 beta Immunogen: Recombinant protein of human HIF1 beta Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ARNT Alternative Names: BHLHE2; Aryl hydrocarbon receptor nuclear translocator; ARNT protein; Class E basic helix-loop-helix protein 2; bHLHe2; Dioxin receptor nuclear translocator; Hypoxia-inducible factor 1-beta; HIF-1-beta; HIF1-betaEntrez Gene (Human): 405Entrez Gene (Mouse) : 11863Entrez Gene (Rat) : 25242SwissProt (Human): P27540SwissProt (Mouse) : P53762SwissProt (Rat) : P41739Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HIF1 beta expression in Raw264.7 (A), NIH3T3 (B), Hela (C), CHOK1 (D), C6 (E), Ramos (F) whole cell lysates.
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Immunohistochemical analysis of HIF1 beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.