Description: Rabbit monoclonal antibody to CD142 Immunogen: A synthetic peptide of human Tissue Factor Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: F3 Alternative Names: Tissue factor; TF; Coagulation factor III; Thromboplastin; CD142Entrez Gene (Human): 2152Entrez Gene (Mouse) : 14066SwissProt (Human): P13726SwissProt (Mouse) : P20352SwissProt (Rat) : P42533Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD142 expression in Hela (A), A549 (B), MCF7 (C), U251 (D), HL60 (E), mouse brain (F), rat brain (G) whole cell lysates.
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  Immunohistochemical analysis of CD142 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.