Description: Rabbit monoclonal antibody to Vinculin Immunogen: Recombinant protein of human Vinculin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: VCL Alternative Names: Vinculin; Metavinculin; MVEntrez Gene (Human): 7414Entrez Gene (Mouse) : 22330Entrez Gene (Rat) : 305679SwissProt (Human): P18206SwissProt (Mouse) : Q64727SwissProt (Rat) : P85972Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Vinculin expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
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  Immunohistochemical analysis of Vinculin staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.