Description: Rabbit monoclonal antibody to NR1D1 Immunogen: A synthetic peptide of human NR1D1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: NR1D1 Alternative Names: EAR1; HREV; THRAL; Nuclear receptor subfamily 1 group D member 1; Rev-erbA-alpha; V-erbA-related protein 1; EAR-1Entrez Gene (Human): 9572Entrez Gene (Mouse) : 217166Entrez Gene (Rat) : 252917SwissProt (Human): P20393SwissProt (Mouse) : Q3UV55SwissProt (Rat) : Q63503Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of NR1D1 expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
• 
  Immunofluorescent analysis of NR1D1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.