Description: Rabbit monoclonal antibody to CD49e Immunogen: A synthetic peptide of human Integrin alpha 5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: ITGA5 Alternative Names: FNRA; Integrin alpha-5; CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5; CD49eEntrez Gene (Human): 3678Entrez Gene (Mouse) : 16402SwissProt (Human): P08648SwissProt (Mouse) : P11688Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD49e expression in K562 (A), NIH3T3 (B) whole cell lysates.
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  Immunohistochemical analysis of CD49e staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.