Description: Rabbit monoclonal antibody to HSF1 Immunogen: A synthetic peptide of human HSF1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: HSF1 Alternative Names: HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1Entrez Gene (Human): 3297SwissProt (Human): Q00613Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HSF1 expression in Hela (A), Jurkat (B) whole cell lysates.
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Immunohistochemical analysis of HSF1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.