Description: Rabbit monoclonal antibody to Hexokinase 2 Immunogen: Recombinant protein of human Hexokinase II Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: HK2 Alternative Names: Hexokinase-2; Hexokinase type II; HK II; Muscle form hexokinaseEntrez Gene (Human): 3099Entrez Gene (Rat) : 25059SwissProt (Human): P52789SwissProt (Rat) : P27881Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Hexokinase 2 expression in rat skeletal muscle (A), Hela (B), CHOK1 (C) whole cell lysates.
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Immunohistochemical analysis of GRP78 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.