Description: Rabbit monoclonal antibody to Glucocorticoid Receptor Immunogen: Recombinant protein of mouse Glucocorticoid Receptor Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: Nr3C1 Alternative Names: GRL; Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1Entrez Gene (Human): 14815Entrez Gene (Rat) : 24413SwissProt (Human): P04150SwissProt (Mouse) : P06537SwissProt (Rat) : P06536Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Glucocorticoid Receptor expression in rat skeletal muscle (A), K562 (B), NIH3T3 (C), Hela (D) whole cell lysates.
-
Immunofluorescent analysis of Glucocorticoid Receptor staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.