Description: Rabbit monoclonal antibody to FKBP4 Immunogen: A synthetic peptide of human FKBP52 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: FKBP4 Alternative Names: FKBP52; Peptidyl-prolyl cis-trans isomerase FKBP4; PPIase FKBP4; 51 kDa FK506-binding protein; FKBP51; 52 kDa FK506-binding protein; 52 kDa FKBP; FKBP-52; 59 kDa immunophilin; p59; FK506-binding protein 4; FKBP-4; FKBP59; HSP-binding immunophilin; HBI; Immunophilin FKBP52; RotamaseEntrez Gene (Human): 2288Entrez Gene (Mouse) : 14228Entrez Gene (Rat) : 260321SwissProt (Human): Q02790SwissProt (Mouse) : P30416SwissProt (Rat) : Q9QVC8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FKBP4 expression in Raw264.7 (A), Jurkat (B), C6 (C), CHOK1 (D), Hela (E) whole cell lysates.
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  Immunofluorescent analysis of FKBP4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.