Description: Rabbit monoclonal antibody to 4EBP1 Immunogen: A synthetic peptide of human eIF4EBP1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: EIF4EBP1 Alternative Names: Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-IEntrez Gene (Human): 1978Entrez Gene (Mouse) : 13685Entrez Gene (Rat) : 116636SwissProt (Human): Q13541SwissProt (Mouse) : Q60876SwissProt (Rat) : Q62622Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of 4EBP1 expression in mouse heart (A), Jurkat (B), C6 (C), CHOK1 (D) whole cell lysates.
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  Immunohistochemical analysis of 4EBP1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.