Description: Rabbit monoclonal antibody to CREB (Phospho-S133) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S133 of human CREB protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: CREB1 Alternative Names: Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1Entrez Gene (Human): 1385Entrez Gene (Rat) : 81646SwissProt (Human): P16220SwissProt (Rat) : P15337Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CREB (Phospho-S133) expression in C6 UV treated (A) whole cell lysates.
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  Immunohistochemical analysis of CREB (Phospho-S133) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.