Description: Rabbit monoclonal antibody to PGHS-2 Immunogen: A synthetic peptide of human COX2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: PTGS2 Alternative Names: COX2; Prostaglandin G/H synthase 2; Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2Entrez Gene (Human): 5743Entrez Gene (Mouse) : 19225Entrez Gene (Rat) : 29527SwissProt (Human): P35354SwissProt (Mouse) : Q05769SwissProt (Rat) : P35355Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PGHS-2 expression in rat brain (A) whole cell lysates.
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Immunohistochemical analysis of PGHS-2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.