Description: Rabbit monoclonal antibody to Beta-tubulin Immunogen: A synthetic peptide of human beta Tubulin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: TUBB Alternative Names: TUBB5; Tubulin beta chain; Tubulin beta-5 chainEntrez Gene (Human): 203068Entrez Gene (Mouse) : 22154Entrez Gene (Rat) : 29214SwissProt (Human): P07437SwissProt (Mouse) : P99024SwissProt (Rat) : P69897Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta-tubulin expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
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Immunohistochemical analysis of Beta-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.