Description: Rabbit monoclonal antibody to BAG6 Immunogen: A synthetic peptide of human BAT3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100) Gene Symbol: BAG6 Alternative Names: BAT3; G3; Large proline-rich protein BAG6; BAG family molecular chaperone regulator 6; BCL2-associated athanogene 6; BAG-6; BAG6; HLA-B-associated transcript 3; Protein G3; Protein ScytheEntrez Gene (Human): 7917Entrez Gene (Mouse) : 224727Entrez Gene (Rat) : 94342SwissProt (Human): P46379SwissProt (Mouse) : Q9Z1R2SwissProt (Rat) : Q6MG49Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of BAG6 expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D) whole cell lysates.
-
Immunofluorescent analysis of BAG6 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.