Description: Rabbit monoclonal antibody to ATG5 Immunogen: A synthetic peptide of human APG5L/ATG5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: ATG5 Alternative Names: APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific proteinEntrez Gene (Human): 9474Entrez Gene (Mouse) : 11793SwissProt (Human): Q9H1Y0SwissProt (Mouse) : Q99J83SwissProt (Rat) : Q3MQ06Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ATG5 expression in Raw264.7 (A), K562 (B), C6 (C), Hela (D) whole cell lysates.
• 
  Immunofluorescent analysis of ATG5 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.