Description: Rabbit monoclonal antibody to Smooth Muscle Actin Immunogen: A synthetic peptide of human alpha smooth muscle Actin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/3000), IH (1/50 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: ACTA2 Alternative Names: ACTSA; ACTVS; Actin aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 proteinEntrez Gene (Human): 59Entrez Gene (Mouse) : 11475Entrez Gene (Rat) : 81633SwissProt (Human): P62736SwissProt (Mouse) : P62737SwissProt (Rat) : P62738Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Smooth Muscle Actin expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
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  Immunohistochemical analysis of Smooth Muscle Actin staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of alpha Smooth Muscle Actin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).