Description: Rabbit monoclonal antibody to SOX9 Immunogen: Recombinant protein of human SOX9 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: SOX9 Alternative Names: Transcription factor SOX-9Entrez Gene (Human): 6662Entrez Gene (Mouse) : 20682SwissProt (Human): P48436SwissProt (Mouse) : Q04887Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SOX9 expression in NIH3T3 (A) whole cell lysates.
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  Immunohistochemical analysis of SOX9 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.