Description: Rabbit monoclonal antibody to Beta-catenin Immunogen: A synthetic peptide of human beta Catenin Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: CTNNB1 Alternative Names: CTNNB; Catenin beta-1; Beta-cateninEntrez Gene (Human): 1499Entrez Gene (Mouse) : 12387Entrez Gene (Rat) : 84353SwissProt (Human): P35222SwissProt (Mouse) : Q02248SwissProt (Rat) : Q9WU82Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Beta-catenin expression in mouse brain (A), C6 (B), CHOK1 (C), Hela (D) whole cell lysates.
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Immunohistochemical analysis of Beta-catenin staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.