Description: Rabbit monoclonal antibody to HMGB1 Immunogen: A synthetic peptide of human HMGB1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: HMGB1 Alternative Names: HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1Entrez Gene (Human): 3146Entrez Gene (Mouse) : 100862258; 15289Entrez Gene (Rat) : 25459SwissProt (Human): P09429SwissProt (Mouse) : P63158SwissProt (Rat) : P63159Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HMGB1 expression in K562 (A), rat brain (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates.
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  Immunohistochemical analysis of HMGB1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.