Description: Rabbit monoclonal antibody to BAX Immunogen: A synthetic peptide of human Bax Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: BAX Alternative Names: BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4Entrez Gene (Human): 581Entrez Gene (Mouse) : 12028SwissProt (Human): Q07812SwissProt (Mouse) : Q07813SwissProt (Rat) : Q63690Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BAX expression in C6 (A), NIH3T3 (B), Hela (C) whole cell lysates.
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  Immunohistochemical analysis of BAX staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.