Description: Rabbit monoclonal antibody to GNB2 Immunogen: A synthetic peptide of human GNB2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/10 - 1/50) Gene Symbol: GNB2 Alternative Names: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2; G protein subunit beta-2; Transducin beta chain 2Entrez Gene (Human): 2783Entrez Gene (Mouse) : 14693Entrez Gene (Rat) : 81667SwissProt (Human): P62879SwissProt (Mouse) : P62880SwissProt (Rat) : P54313Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GNB2 expression in rat brain (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.
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Immunohistochemical analysis of GNB2 staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.