Description: Mouse monoclonal antibody to CAPN6 Immunogen: Recombinant fusion protein of human CAPN6. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50) Gene Symbol: CAPN6 Alternative Names: CALPM; CANPX; Calpain-6; Calpain-like protease X-linked; Calpamodulin; CalpMEntrez Gene (Mouse) : 12338SwissProt (Mouse) : O35646Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of CAPN6 expression in human placenta (A) whole cell lysates.
-
Flow cytometric analysis of A549 cells using Anti-CAPN6 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.