Anti-RAB23 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of RAB23 expression in human brain (A), mouse brain (B), MDAMB231 (C), mouse Cerebellum (D), human Cerebellum (E) whole cell lysates.
Immunofluorescent analysis of RAB23 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of U2OS cells using Anti-RAB23 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Product Name	Anti-RAB23 Antibody
Cat No:	CQA9543
Source:	Mouse
Reactivity:	H, M
Applications:	WB, IF/IC, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

50 μl

$150

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Description: Mouse monoclonal antibody to RAB23

Immunogen: Recombinant fusion protein of human RAB23. The exact sequence is proprietary.

Purification: This antibody is purified through a protein G column.

Clonality: Monoclonal

Form: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)

Gene Symbol: RAB23

Alternative Names: Ras-related protein Rab-23

Entrez Gene (Human): 51715

SwissProt (Human): Q9ULC3

SwissProt (Mouse) : P35288

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of RAB23 expression in human brain (A), mouse brain (B), MDAMB231 (C), mouse Cerebellum (D), human Cerebellum (E) whole cell lysates.
Immunofluorescent analysis of RAB23 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of U2OS cells using Anti-RAB23 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

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