Description: Mouse monoclonal antibody to GLS2 Immunogen: Recombinant fusion protein of human GLS2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GLS2 Alternative Names: GA; Glutaminase liver isoform, mitochondrial; GLS; L-glutaminase; L-glutamine amidohydrolaseEntrez Gene (Human): 27165Entrez Gene (Mouse) : 216456SwissProt (Human): Q9UI32SwissProt (Mouse) : Q571F8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GLS2 expression in human brain (A), mouse liver (B) whole cell lysates.
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Immunohistochemical analysis of GLS2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.