Description: Mouse monoclonal antibody to MBD2 Immunogen: Recombinant fusion protein of human MBD2. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MBD2 Alternative Names: Methyl-CpG-binding domain protein 2; Demethylase; DMTase; Methyl-CpG-binding protein MBD2Entrez Gene (Human): 8932SwissProt (Human): Q9UBB5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MBD2 expression in K562 (A), MCF7 (B), Jurkat (C) whole cell lysates.
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Immunohistochemical analysis of MBD2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.