Description: Mouse monoclonal antibody to ATG3 Immunogen: Recombinant fusion protein of human ATG3. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ATG3 Alternative Names: APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96Entrez Gene (Human): 64422Entrez Gene (Mouse) : 67841SwissProt (Human): Q9NT62SwissProt (Mouse) : Q9CPX6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATG3 expression in THP1 (A), mouse liver (B), mouse testis (C) whole cell lysates.
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  Immunohistochemical analysis of ATG3 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.