Anti-RAB18 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of RAB18 expression in PC3 (A), mouse brain (B), rat brain (C), human brain (D), human testis (E) whole cell lysates.
Flow cytometric analysis of PC3 cells using Anti-RAB18 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Product Name	Anti-RAB18 Antibody
Cat No:	CQA9513
Source:	Mouse
Reactivity:	H, M, R
Applications:	WB, FC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

50 μl

$150

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Description: Mouse monoclonal antibody to RAB18

Immunogen: Recombinant fusion protein of human RAB18. The exact sequence is proprietary.

Purification: This antibody is purified through a protein G column.

Clonality: Monoclonal

Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), FC (1/10 - 1/50)

Gene Symbol: RAB18

Alternative Names: Ras-related protein Rab-18

Entrez Gene (Human): 22931

Entrez Gene (Mouse) : 19330

Entrez Gene (Rat) : 307039

SwissProt (Human): Q9NP72

SwissProt (Mouse) : P35293

SwissProt (Rat) : Q5EB77

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of RAB18 expression in PC3 (A), mouse brain (B), rat brain (C), human brain (D), human testis (E) whole cell lysates.
Flow cytometric analysis of PC3 cells using Anti-RAB18 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

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