Description: Mouse monoclonal antibody to NANOG Immunogen: Recombinant fusion protein of human NANOG. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column. Clonality: Monoclonal Form: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/1000 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NANOG Alternative Names: Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanogEntrez Gene (Human): 79923SwissProt (Human): Q9H9S0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of NANOG expression in Caco2 (A), MCF7 (B) whole cell lysates.
• 
  Immunohistochemical analysis of NANOG staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.